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Non-bleaching Fluorescent Probes


Flow cytometry has become a powerful tool for the identification of specific cell populations. Cellular phenotyping is accomplished by staining cells with a fluorescently labeled antibody specific for a specific cell marker. However, if the antibody is not properly developed or poorly labeled with a weak fluorescent tag, percentage of cells may not be accurately determined. Moreover, during processing, if the labeled antibody is exposed to light, fluorescent probes bleach altering results. 

IQFCS has developed highly specific antibodies labeling them with a non-bleaching fluorescent label. IQFCS offers multiple antibodies directly labeled with AQ488 (FL-1) or AQ647 (FL-3) for easy cell identification.



Bleaching2.jpg

 

Figure 1

Figure 1. Human PBMCs were stained with 2 different commercially available human CD3-FITC or anti-CD3-AQ488 labeled antibodie and placed under a fluorescent light. Samples were removed every 15 minutes and analyzed by flow cytometry. Both commercial antibodies rapidly bleached altering percentage of CD3 positive lymphocytes. Cells stained with hCD3-AQ488 retained its fluorescent intensity.

 

Isotype Control
Isotype.jpg
Figure 2

 

h-CD4-AQ488
AQ488.jpg
Figure 3

 

h-antiCD8-AQ647
AQ647.jpg
Figure 4.

Total PBMCs were stained with isotype control (Fig 2), anti-CD4-AQ488 (Fig 3), or anti-CD8-AQ647 (Fig 4). Antibodies conjugates with AQ488 or 647 allow for clear bright separation from negative population (Fig 3) making clear quantatable results. Moreover, the clear bright separation allows researchers to clearly distinguish low and high populations (Fig 4).

Available antibodies labeled with AQ-488. For pricing information, please request a quote.

PRODUCT CODE
ANTIBODIES
TEST SIZE
Iso488150
Isotype-AQ488
125
Iso488250
Isotype-AQ488
250
h3488125
anti-hCD3-AQ488
125
h3488250
anti-hCD3-AQ488
250
h4488125
anti-hCD4-AQ488
125

h4488250

anti-hCD4-AQ488

250

h8488125

anti-hCD8-AQ488

125

h8488250

anti-hCD8-AQ488

250

Ig488125

anti-IgG-AQ488

125

Ig488250

anti-IgG-AQ488

250

hm488125

anti-hm-AQ488

125

hm488250

anti-hm-AQ488

250

Available antibodies labeled with AQ-647. For pricing information, please request a quote.

PRODUCT CODE
ANTIBODIES
TEST SIZE
Iso647150
Isotype-AQ647
125
Iso647250
Isotype-AQ647
250
h3647125
anti-hCD3-AQ647
125
h3647250
anti-hCD3-AQ647
250
h4647125
anti-hCD4-AQ647
125

h4647250

anti-hCD4-AQ647

250

h8647125

anti-hCD8-AQ647

125

h8647250

anti-hCD8-AQ647

250

Ig647125

anti-IgG-AQ647

125

Ig647250

anti-IgG-AQ647

250

hm647125

anti-hm-AQ647

125

hm647250

anti-hm-AQ647

25

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AQ Molecular and Immunochemical