Total Cytotoxicity Assays

Determining the specific mechanism of cellular death is important for the prevention of infectious disease.  In a single tube, scientists can differentiate apoptosis from necrosis to during a cytolytic response.  

ASSAY PRINCIPLE:
All target cells are labeled with CFSE.  Unstained effectors cells are added and co-incubated with the target cells.  Adding a poly caspase inhibitor that will bind to active caspases within the cell can identify cells that are undergoing apoptosis.  After incubation, a live-dead stain is added to identify those cells with a compromised cell membrane (dead).   As shown in the dot plot, cells undergoing
EARLY apoptosis are positive with the caspase probe but are not yet detected as “dead” by 7-AAD. The IQFCS kit provides the most sensitive assay available to assess cytotoxicity.


EACH KIT INCLUDES:
Kit size choice of Tcy125, Tcy250 or Tcy500 tests
Reagents include:
• CFSE (a green membrane stain)
• Poly caspase inhibitor FMK-VAD-SR (detect apoptosis)
• 7-AAD (live dead DNA stain)