ASSAY PRINCIPLE:
All target cells are labeled with CFSE. Unstained effectors cells are added and co-incubated with the target cells. Adding a poly caspase inhibitor that will bind to active caspases within the cell can identify cells that are undergoing apoptosis. After incubation, a live-dead stain is added to identify those cells with a compromised cell membrane (dead). As shown in the dot plot, cells undergoing
EARLY apoptosis are positive with the caspase probe but are not yet detected as “dead” by 7-AAD. The IQFCS kit provides the most sensitive assay available to assess cytotoxicity.

EACH KIT INCLUDES:
Kit size choice of Tcy125, Tcy250 or Tcy500 tests
Reagents include:
• CFSE (a green membrane stain)
• Poly caspase inhibitor FMK-VAD-SR (detect apoptosis)
• 7-AAD (live dead DNA stain)