American Qualex - Manufacturing Quality and Excellence

Secondary Antibodies

American Qualex and its partner companies provide the highest quality secondary antibodies in the industry.

Adsorbed antibodies are typically adsorbed against immunoglobulins from up to 14 different species.

AMERICAN QUALEX Affinity Purified Secondary Antibodies are tested for purity and specificity by immunoelectrophoresis and immunoprecipitation. Conjugates are tested for immuno-enzyme activity by direct enzyme immunoassay. Even product includes a product data sheet which contains all the pertinent information as well as suggested working dilutions.
Unlabeled Affinity-purified antibodies are supplied at a concentration of 2.0 mg/mi in 0.01 M phosphate buffered saline (PBS), pH 7.6 with 0.1% sodium azide as a preservative. Store at -20 C.

Alkaline Phosphatase (Calf intestine) labeled antibodies are supplied at 0.5 mg/ml in 0.05 M Tris-HCl, ph 8.0 with 0.001 M MgC12, 1% BSA and 0.1% sodium azide. Suggested working dilution is approximately 1:5000 for direct ELISA. Store at 4C.

Horseradish Peroxidase (HRPO) labeled antibodies are provided at 1.0 mg/ml in 0.01 M PBS, pH 7.6 containing 10% mannose, 2% sucrose, and 1% BSA as stabilizing reagents and 0.02% thimerosal as a preservative. Suggested working dilution is approximately 1 :10,000 for direct ELISA. Store at 4C.

Beta-Galactosidase E. coli) labeled antibodies are packaged at 0.25 mg/ml in 0.05 M PBS, pH 7.0 with 1% BSA and 0.1% sodium azide. Suggested working dilution for fluorescent EIA is approximately 1:3,000, for ELISA 1:1,000. Store at 4C.

Enzyme Immune Adsorbant Assays (ELISA), Staining, and Blotting applications require conjugates, that are high in sensitivity, specificity, and working dilution. AQ offers antibodies labeled with Horseradish Peroxidase, Alkaline Phosphatase, and Beta-Galactosidase. Each enzyme is conjugated to antibodies using the method that will best optimize its performance. AQ features a bidirectional method of conjugating Alkaline Phosphatase and Peroxidase. This procedure yields a highly sensitive conjugate that helps in retaining optimal antibody and enzyme activity for assays done on solid support, such as polystyrene or nitrocellulose . Whole molecules and F(ab')2 fragments are available cross-adsorbed for species specificity, ideal for species sensitive assays.

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AQ Molecular and Immunochemical