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Apoptosis vs. Necrosis Assays

Understanding the mechanisms involved in cellular death is important for vaccine and pharmaceutical drug safety and efficacy. Cells undergo death by two possible routes.   
• A programmed process known as APOPTOSIS or
• Direct lysis of the cell membrane known as NECROSIS.
In this example, the mechanisms of cell death initiated by two different proteins were examined. Either protein A or B was incubated with an epithelial cell line for 24 hours. Thirty minutes before analysis, a cell permeant apoptosis probe was added. Cells were then released from the plate; a live-dead stain was introduced and analyzed by flow cytometry.  A necrotic response was induced by protein A while protein B initiated apoptosis.  This assay allows the scientist to easily determine which mechanism a compound uses to cause cellular death.
 
KIT INCLUDES:
Kit sizes available -- ANDS125, ANDS250, ANDS500 or ANDS1000 tests
• 7-AAD live dead stain
• FAM-VAD-FMK stain (polycaspase conjugated to fluorescein 488)
• Assay buffer and manual 
 

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AQ Molecular and Immunochemical