• A programmed process known as APOPTOSIS or
• Direct lysis of the cell membrane known as NECROSIS.
In this example, the mechanisms of cell death initiated by two different proteins were examined. Either protein A or B was incubated with an epithelial cell line for 24 hours. Thirty minutes before analysis, a cell permeant apoptosis probe was added. Cells were then released from the plate; a live-dead stain was introduced and analyzed by flow cytometry. A necrotic response was induced by protein A while protein B initiated apoptosis. This assay allows the scientist to easily determine which mechanism a compound uses to cause cellular death.
KIT INCLUDES:
Kit sizes available -- ANDS125, ANDS250, ANDS500 or ANDS1000 tests
• 7-AAD live dead stain
• FAM-VAD-FMK stain (polycaspase conjugated to fluorescein 488)
• Assay buffer and manual
