• FLIVO is a direct stain that enters the cells of living animals upon injection
• Analysis of tissues is conducted through a window chamber, fluorescent microscope or by flow cytometry.
• Unbound FLIVO reagent is removed from the cells by natural circulation
In vivo labeling of caspase-positive neurons reveals a remarkable difference in the extent of apoptotic cell death in the nucleus magnocellularis (NM) of control (left) and cochlear removal (right) from bird brains after the injection of FAM-FLIVO.
EACH KIT INCLUDES:
FLIVO probes are available as red sulforhodamine (SR), or green carboxyfluorescein,
(FAM) conjugates with enough reagents to test 6 or 24 mice.
• FLIVO was developed by Immunochemistry Technologies, Bloomington, MN
Manual
References
Griffin
, R.J., Williams, B.W., Loren, M., Bischof, J.C., Olin, M., Lee, B.W.,
and Johnson, G. 2006. Novel use of highly specific poly-caspase
inhibitor for in vivo detection of apoptosis in tumors treated with
vascular targeting agents. Poster presented at the American Association
for Cancer Research, April 2006.
Griffin
, R., Williams, B.W., Olin, M., Lowen, M., and Song, C.W. 2005.
Thermoradiotherapy enhancement with anti-vascular strategy. Poster
presented at the Radiation Research Society, October 17, 2005. Morrow,
T., Paulson, E. October 16, 2006. Diabetes-induced allodynia:
correlation between behavioral intensity, duration of diabetes, and
periaqueductal gray (PAG) activation. Poster presented at the Society
for Neuroscience annual meeting, October 2006.
Olin,
M., Roy , S., Peterson, P., and Moliter, T. 2006. In vivo model for the
detection of morphine-induced apoptosis. Poster presented at the
Society of Neuroimmunology Pharmacology (SNIP), April, 2006.
